Fig. 4. The spatial distribution of M cells and dome enterocytes is already established at the base of domes from rabbit appendix and Peyer’s patch. Micro-dissected domes from Peyer’s patch (A-C) and appendix (D) were permeabilized and co-labeled with MAb 58 (green) and MAb 214 (red in A, B, D) or anti-vimentin (red in C). (A) The regular mosaic distribution pattern of M cells and enterocytes, already established at the very base of the dome around a crypt region. (B) A higher magnification reveals marked variations in M cell apical surfaces (thin and open arrows). (C) is the maximal projection of 60 focal planes to localize both the apical surface of M cells and the lateral corresponding pocket: note that M cells with large apical surfaces (open arrows) correspond to large vimentin-delimited pockets, while M cells with compressed apical surfaces (thin arrows) correspond to small pockets. (D) The relative distribution of M cells and enterocytes in appendix reveals a very regular mosaic pattern from the base to the upper part of the dome. A few goblet cells were double labeled in yellow at the lower part of the domes (arrows).
