Fig. 5. The N-terminal domain of pEg2 fused to GFP localises to the centrosome in XL2 cells. GFP, Nt-pEg2-GFP and Cd-pEg2-GFP proteins were constitutively expressed in Xenopus XL-2 cells using transient transfection with the pEGFPN1 expression vector. Cells were fixed on coverslips and processed for immunofluorescence. (A-D) GFP transfected cells (control), (E-H) Nt-pEg2-GFP transfected cells, (I-L) Dc-pEg2-GFP transfected cells. (A,E,I) Hoechst-stained DNA. (B,F,J) 
-tubulin staining with a rabbit polyclonal antibody (diluted 1:1000), revealed with a Texas Red-conjugated secondary antibody (diluted 1:500). (C,G,K) The localisation of the GFP proteins. (D,H,L) Overlay. Scale bar, 10 µm. The localisation of the proteins was observed by fluorescence microscopy (DMRXA Leica); the images were acquired using a black and white camera and treated with the Leica-Q-Fish program
