Fig. 3. P32-myc localisation in mitochondria is blocked by N-terminal residues. Cells were transfected with p32-myc (panel a) and flag-p32-myc (panel b) and stained with anti-prohibitin (red) and anti-myc antibodies (green). The individual images are shown merged in the right-hand panel. Arrows indicate cells transfected expressing the p32 species. p32-myc and prohibitin showed almost complete co-localisation, appearing yellow in the merged image, whereas the flag-p32 appears in a distinct globular pattern showing little overlap with the mitochondria.
