Fig. 10. MMP-2 activation in Sertoli cells co-cultured with germ cells. (A) Western blot analysis of TIMP-2 in Sertoli cells (Sc), peritubular cells (Pc) and crude germ cells (CGc). 40 µg of proteins were deposited per lane on 15% SDS-PAGE. (B) Western blot analysis of MMP-2 in Sertoli cells (Sc) and in Sertoli cells co-cultured with crude germ cells (CGc), spermatocytes (SPC) or spermatids (SPT). 40 µg of proteins were analyzed per lane on 7.5% SDS-PAGE. (C) Sertoli/crude germ cells co-culture media were analyzed by gelatin zymography. 80 µg of proteins were analyzed per lane in lanes 1 and 40 µg in lanes 2. The sizes of the lytic bands are indicated on the right.
