Fig. 4. Analysis of MT1-MMP gene expression during testicular development and in purified testicular cells by RT-PCR (A,B). Total RNA was prepared from (A) whole testis at various ages (17.5 dpc and 1, 10, 20, and 60 days old) and (B) from adult rat liver (positive control), Sertoli cells (Sc), peritubular cells (Pc), Leydig cells (Lc), spermatocytes (SPC), spermatids (SPT) and crude germ cells (CGc). The expected size of the PCR product is 329 bp. A DNA ladder has been included in the gels. (C) Northern blot analysis of MT1-MMP in 10-day-old (lane 1) and 15-day-old (lane 2) rat testis, in cultured Sertoli (lanes 3, 4) or peritubular cells (lane 5), and in crude germ cells (lane 6), using total RNA (10-20 µg) except in lane 3 (3 µg of polyA(+)). The positions of the 28S and 18S are indicated.
