Fig. 8. FSH and TIMP-2 gene expression. Sertoli cells were treated or not treated with increasing doses of FSH ranging from 0.1 ng ml-1 to 100 ng ml-1, after which total RNA was extracted and 20 µg were analyzed by northern blots (A). Blots were hybridized successively with the TIMP-2 and 18S probes, and quantified. A representative blot is shown in the inset (lanes 1-6 correspond to cultures exposed to 0-100 ng ml-1 FSH for 48 hours). Data are expressed as mean±s.e.m. of triplicate dishes; control is lane 1, with no FSH. P value against the control: *, 0.05. (B) TIMP-2 antigen levels were analyzed by western blot in the culture media of Sertoli cells treated with (+) or without (-) FSH for 24, 48 or 72 hours.
