Fig. 9. Gelatin zymography of Sertoli cell culture media (A) and the action of FSH (B). (A) Purified human MMP-2 (lane 1, 1 ng) and Mr standards (lane 3) were used to determine the size of the lytic bands present in a 3-day-old Sertoli cell culture media (lane 2). (B) Sertoli cells were treated with FSH (50 ng/ml) (+) or not treated (-) for 24, 48 and 72 hours, after which culture medium was collected and 40 µg of proteins were analyzed by gelatin zymography. The sizes of the lytic bands are indicated on the right.
