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Fig. 7. Expression of non-degradable cdc13-des2 destruction box mutant inhibits sporulation. The diploid strain h-/h- pat1-114/pat1-114 (Sp964) was transformed with pJK148-cdc13+ or pJK148-cdc13-des2 to generate the strains Sp969 and Sp970, respectively. Single-copy integrants at the leu1 locus were isolated and induced to undergo a synchronous meiosis. (A) Photographs of cells after 9 hours at 34°C. (B) Percentage of asci with 4, 3, 2, 1 and 0 spores.





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