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Fig. 3. ClC-3 channels localize to the B-cell granule membrane. (A) Distribution of anti-hClC-3c binding, insulin immunoreactivity and overlay of anti-hClC-3c and insulin immunoreactivity visualized by confocal immunocytochemistry. Bar, 2 µm. (B) Immunogold detection of ClC-3 localization to insulin-containing secretory granules (SG) visualized by electron microscopy. The 12 nm gold particles appear as small black dots (arrow). Bar, 100 nm. Ly, lysosome.





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