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Fig.2 Movie of fluorescence images of an Ax2 cell with an extending protrusion. Cells had been stained with RH-795 10 minutes before quinine application. This dye binds to contractile vacuole membranes with high affinity. Note the highly fluorescent patch in the leading edge of the protrusion.
Fig.4 Movie showing concanavalin A-tagged beads attached to the cell surface during elongation of protrusion. Fluorescence is shown in red and Nomarski images in green.
Fig.6C Movie of fluorescence images of an mhc-null cell showing expulsion of a contractile vacuole. The cell had been stained with RH-795 10 minutes before quinine application. A patch of fluorescence persists on the cell membrane at the site of expulsion of one of the contractile vacuoles.
Fig.7A Dynamics of GFP-myosin II during the formation of a protrusion in the presence of quinine.
Fig.7B Another cell showing a travelling band of GFP-myosin II on the protrusion towards its tip.
Fig.10A Movie of a GFP-ABD cell exposed to quinine.
Fig.10B Movie of a GFP-ABD cell exposed to quinine.
Fig.11 Dynamics of GFP-myosin II during bleb formation in the presence of quinine and cytochalasin A.
Fig.12 Movie of a locomoting slug cell in the absence of quinine. Slugs migrating on agar were dissociated into single cells in phosphate buffer by repeated passage through a 25G needle, and plated on a coverslip in such a way that the buffer formed a thin film of a depth just enough to cover the cells.
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