Fig. 6. c-Cbl and the EGFR colocalize at the plasma membrane in the presence of dominant negative dynamin. The human EGFR and c-Cbl were coexpressed in CHO cells, in combination with either the dynamin K44A mutant (A-F) or wild-type dynamin (G-I). Cells were serum starved and incubated without (A-C) or with 25 ng ml-1 EGF for 5 minutes (D-I). Cells were fixed and stained with antibodies directed against the EGFR (green; A,D,G) and c-Cbl (red; B,E,H) and analysed by confocal microscopy. Merge images are shown in C, F and I. The confocal image in panels G-I was taken at the middle of the cells. Inserts show xz sections.
