Fig. 2. Peroxisomal targeting of PMPs is not inhibited by BFA. Normal human fibroblasts were incubated in either the presence (A-J) or the absence (K,L) of 2 µg ml-1 BFA for 20 minutes and then microinjected with DNA constructs expressing Pex2p-GFP (A,B), Pex3p-GFP (C,D), Pex16p-GFP (E,F), GFP-PMP70 (G,H) and HA (I-L). After microinjection, the cells were maintained in mock or BFA-containing media for 90 minutes and then processed for double indirect immunofluorescence with antibodies to GFP (A,C,E,G) and ALDP (B,D,F,H), or with antibodies to HA (I,K) and PDI (J,L).
