Fig. 3. Apico-basal polarity is affected in let-413(RNAi) but not dlg-1(RNAi) embryos. (A-F) Confocal analysis showing the internal focal plane of embryos after immunostaining with anti-PAR-3 (A-C) or anti-PAR-6 (D-F) antibodies alone, or along with anti-HMP-1 antibodies (A'-C' and D'-F', respectively). (A'-F') Regions within the dotted box in A-F, respectively, at over 2x magnification. (G,H) Most external focal plane, (I) more internal focal plane within epidermis, showing GFP fluorescence of che-14::gfp transgenic embryos. (A,D,G) Wild-type embryos; (B,E,H) dlg-1(RNAi) embryos; (C,F,I) let-413(RNAi) embryos. In this and all following figures, dlg will refer to dlg-1(RNAi) embryos and let-413 will indicate let-413(RNAi) embryos. Apical markers of the epidermis and intestine are mislocalised in let-413(RNAi) embryos while the same markers remain apical to or coincidental with HMP-1 in dlg-1(RNAi) embryos; note that there are regions of lateral CHE-14::GFP expression in dlg-1(RNAi) embryos (arrows in H) and that CHE-14::GFP was not visible in the most external focal plane of let-413(RNAi) embryos. The intestinal lumen is wider in dlg-1(RNAi) embryos than in wild-type embryos (compare A,D with B,E). (J) Diagram illustrating the general localisation of PAR-3, PAR-6 and CHE-14 (green) in a hypothetical epithelial cell in wild-type, dlg-1(RNAi) and let-413(RNAi) embryos. Scale bars, 10 µm (A-I); 5 µm (A'-F').
