Fig. 5. CeAJ proteins are spread along the lateral membrane in differentiating epidermal cells of wild-type embryos. (A) Diagram of an early wild-type embryo (at the time of epidermal differentiation) showing a dorsal view representing embryos in images B-E. (A') Schematic transverse section across an epidermal cell corresponding to the blue bracket in A and representing images B'-E'. (B-E) Confocal analysis showing external focal plane projections of wild-type (B,D) and dlg-1::gfp transgenic (C,E) embryos at a stage of development prior to (B), and coincidental with (C-E), epidermal cell differentiation, after immunostaining with anti-HMP-1 (B,C), anti-LIN-26 (B), MH27 (anti-JAM-1) (D,E), anti-GFP (C,E) and anti-UNC-70 (D) antibodies and DAPI (D). UNC-70 is used here and elsewhere as a general membrane marker. (B'-E') Z-axis rotations of a single transverse slice through a section of the embryo as denoted by the corresponding lines in embryos B-E, showing CeAJ markers distributed along the lateral membranes. Apical is to the right in all Z-axis rotations here and elsewhere. (C') Reveals the mutually exclusive nature of DLG-1 and HMP-1 localisation and (E') illustrates that DLG-1 and JAM-1 exhibit complete co-localisation. Asterisks in B' and D' indicate the position of epidermal nuclei. (F) A cartoon showing the position of membrane-associated proteins in immature and mature epidermal cells. CeAJ proteins are positioned along the membrane in immature epidermal cells and relocate to a compact subapical junction as the cells mature (see Fig. 6). Scale bars, 10 µm.
