Fig. 4. 1.12 and 2.52 coimmunoprecipitate with GalT. (A) Lysates from cells stably transfected with GFP-1.12 were immunoprecipitated (Ip) with rabbit preimmune sera (PI) or sera raised against recombinant GalT (I) in the presence or absence of calcium, magnesium or phosphatidyl serine (PS) and immunoblotted with GFP antibodies. (B) Lysates from cells stably transfected with BFP-2.52 were immunoprecipitated with preimmune sera (PI) or antibodies against GalT (I) and immunoblotted with GFP antibodies. In both A and B the electrophoretic mobility of the immunoprecipitated GFP/BFP fusion protein (arrows) was determined by immunoblotting samples of the cell lysates (L) with anti-GFP antibodies. (C) Lysates from GFP-1.12 and BFP-2.52 expressing cells were immunoprecipitated with either control sera (PI) or antibodies against GFP (I), resolved under non-reducing conditions, and immunoblotted with antibodies against GalT. Molecular weight markers are shown to the left of each panel.
