Fig. 2. Bfa1 and Bub2 physically associate with Tem1. pGAL-6HIS-TEM1 was introduced into strain SLY106 (BUB2-13MYC 3HA-BFA1) and control strain CG378. Mid-log cells (ML) grown with or without galactose (lanes 1-4) were sampled. The culture of strain SLY106 with galactose was divided and 
-factor (F), HU or nocodazole (Noco) added and incubation continued for 3 hours before sampling. Crude extracts were prepared and incubated with nickel beads. Proteins binding to the beads were eluted and an immunoblot prepared. This was probed with the antibodies shown. Note that strain CG378 is congenic, not isogenic, with strain SLY106, which may account for the high level of Tem1 in lane 1.
