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Fig. 6. CHMP1 subnuclear domains are nuclease-resistant. IND-CHMP1 cells were induced for 24 hours with muristerone, fixed and digested with the indicated amount of DNaseI for 1 hour at room temperature as described (Nickerson et al., 1997). Nuclei were double-labeled for CHMP1 or phosphorylated (Ser10) histone H3 (P-H3), and DNA (Hoechst 33258) and visualized by fluorescence microscopy.





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