Fig. 1. FasL is differentially sorted in haematopoietic cells with secretory lysosomes. FasLWT-GFP was transiently expressed in the non-haematopoietic cell lines, HeLa (A-C) and Rat-1 (D-F) and the haematopoietic cell lines, WR19L (G-I) and RBL (J-L). 36 hours post-transfection, the cells were fixed and co-stained for the lysosomal membrane proteins Lamp-1 (A,G) or Lgp-120 (D,J) and analysed by confocal microscopy. The images were merged (C,F,I,L) to demonstrate co-localisation of FasL and Lamp-1/Lgp120 in the lysosomal compartment. Bars, 10 µm except G-I, 5 µm.
