Fig. 3. Colocalization of SCP1 and SCP3 on the univalent X chromosome. (A-C) Combined SCP3 and SCP1 staining (green and red respectively, with yellow colocalization) and FISH with X-chromosome specific (purple) and telomeric (purple) probes. (D-F) SCP1 staining alone. (A,D) ‘Fully self-synapsed’ X chromosome showing colocalization of SCP3 and SCP1 (yellow) along the length of the SC. (B,E) ‘Asynapsed’ X chromosome showing no evidence of SCP1 staining. (C,F) ‘Asynapsed’ X chromosome showing SCP1 staining along the entire length of the SC. Note: the magnification is the same in all images; however, SC length is dependent upon the stage of the cell (e.g. B,E represent early pachytene) and the synaptic behavior of the X (e.g. the fully self-synapsed X, as in A,D, is half the length of the asynapsed X, as in C,F).
