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Fig. 2. Emergence of the Sad1-containing bodies in the zygotes of strain BG993. (A-C) Chr, DAPI; Sad1, anti-Sad1 antibody; Cut12, GFP-tagged Cut12; Merged: cyan, DAPI; red, Sad1; green, Cut12; overlapped region of Sad1 and Cut12, yellow. Bar, 2.5 µm. (A) 0 hours, (B,C) 4 hours after transferring to the rich medium. (D) Number of cells containing indicated number of the Sad1-bodies per nucleus at the indicated time after transfer.





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