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Fig. 5. Positioning of telomeres and centromeres during RTG in wild-type zygotes of BG991. Chr, DAPI; Tel, FISH with cos212; Cen, FISH with pRS140; Sad1, anti-Sad1 antibody; Merged: blue, DAPI; green, cos212 (in A,B) or pRS140 (in C-F); red, Sad1. (A) 0 hours, (B) 4 hours (C,D) 0 hours, (E,F) 3 hours after transfer. Bars, 2.5 µm.





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