Fig. 2. Disposition of the plasma membrane about the microspheres after 2 minutes (A) and 8 minutes (B) incubation of E-CHO cells with EGF-coated microspheres. Each panel presents one confocal slice of the total image (left) and two sets of enlargements of the microspheres located in the regions-of-interest defined in the overviews. One xy-slice out of a total of 20, and xz- and yz-projections along the indicated lines are shown. Outlines of the morphology of the plasma membrane as derived from the fluorescence images are included. (A) Microspheres, located in an invagination of the plasma membrane reaching down nearly the entire thickness of the cell. In other cases, engulfment was complete at 2 minutes. (B) Microsphere internalized into the cell, concluded from the presence of fluorescence of the outer membrane above the microsphere. Deconvolution was carried out as described in Materials and Methods.
