Fig. 7. Time-dependent correlation of Shc translocation with the activation of EGFR. The data sets from three different experiments were subdivided for low, medium and high levels of Shc in the local environment. The intervals for the subsets were adjusted to roughly equal numbers of events per subset and were the same for all experiments. Values within each subset were smoothed by a moving average with a window size of five to further compensate for the effect of the local Shc concentration on the analysis of receptor-dependent Shc translocation. The arrow indicates those data points lying outside the major population that were caused by a high Shc concentration in the local environment. The values for EGFR activation do not extend below 20 a.u. because of thresholding in the image processing procedure.
