Fig. 1. GFP-Rab7 localizes to early and late phagosomal membranes. GFP-Rab7 expressing cells were pulsed with live yeast for 10 minutes and then fixed with 1% formaldehyde and viewed by fluorescence (A) or DIC (A') microscopy. The arrow denotes an early phagosome ringed with GFP-Rab7. GFP-Rab7 expressing cells were pulsed with live yeast for 120 minutes then fixed with 1% formaldehyde and viewed by fluorescence (B) or DIC (B') microscopy. The arrow denotes a multi-particle phagosome ringed with GFP-Rab7. Bar, 2 µm.
