Fig. 3. Cellular localization of Hsc70 and other molecular chaperones and cochaperones. Serum-starved IMR-90 fibroblasts were fixed in methanol at -20°C. and then incubated with mAb 13D3 followed by FITC-conjugated secondary antibodies, anti-DNA antibodies followed by Cy5-conjugated secondary antibodies, and antibodies against either Hip, Hop, BAG-1 or Hsp40 followed by Texas Red-conjugated secondary antibodies. Incubation with anti-Hsp90 antibodies was followed by Rhodamine-conjugated secondary antibodies. Bar, 10 µm.
