Fig. 5. Inhibition of protein transport into lysosomes with specific antibodies. Lysosomes (100 µg protein) in a total volume of 0.1 ml were incubated with increasing amounts of mouse serum containing anti-Hip, nonimmune mouse serum, rabbit serum containing anti-Hop or anti-Hsp40, or nonimmune rabbit serum as indicated. Samples were treated as described in Materials and Methods, and membranes were probed with either rabbit anti-RNase A or mouse anti-GAPDH, and developed with chemiluminescense (see Materials and Methods). The lower panels are the densitometric quantitation of the results.
