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Fig. 4. PtdIns(4,5)P2 co-localises with SC-35 in interphase cells. Detergent-permeabilised HeLa cells were co-stained with Cy3-2C11 (B,E,H,J,K) and either anti-SC-35 (A), anti-Sm (D) or anti- RNA Pol IIo (H5, G) antibodies. The yellow pseudo-colour (merge; C,F,I) shows the extent of co-localisation between the two antigens. RNase (K), but not DNase I (J) treatment abolishes PtdIns(4,5)P2 staining. Confocal microscopy images corresponding to the projection of a series of 0.4 µm sections are shown. Bars, 10 µm.





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