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Fig. 9. PtdIns(4,5)P2 associates with splicing complexes. Splicing reactions were carried out prior to immunoprecipitation. In these conditions, intermediates and products of the splicing reaction associate with anti-PtdIns(4,5)P2, but not control beads. Pre-incubation of the antibody with GroPIns(4,5)P2 but not GroPIns inhibits the recovery. A positive control immunoprecipitation with Y12 is shown for comparison.





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