Fig. 4. Optical density waves in mounds. Subtracted images of the mounds (A-D) reveal the wave pattern. Due to the image processing the wave fronts appear as black and white bands. Bar, 250 µm. In the schematic representations in the middle row (E-H), waves are shown as bold black lines and the direction of wave propagation is indicated by little arrows. Small circles represent the cores around which the spiral waves rotate, whereas the black dot in H marks the position from which concentric waves originated periodically. The outline of the mounds is indicated by a thin black line. The final phenotype after 24 hours is shown in the bottom row (I-L). (A,E,I) Parental strain DH1 with multi-armed spiral waves. (B,F,J) cAR1 mutant with single-armed spiral wave. (C,G,K) cAR3 mutant with complex wave patterns. (D,H,L) N272 mutant with concentric waves.
