Fig. 1. Expression of chicken ß1 in GD25 cells. Three constructs expressing chicken ß1 with site directed substitutions at S785 of the cytoplasmic domain (A) were transfected into GD25 cells. Biotin surface labeled cells from clones expressing chicken S785S, S785M or S785D ß1 were immunoprecipitated using anti-chicken-ß1 antibody (W1B10) and the precipitates analyzed by SDS-PAGE followed by western blotting probed with streptavidin (B). Biotin surface labeled cells from clones expressing chicken S785S, S785M or S785D ß1 were immunoprecipitated using anti-mouse-
6 antibody (GOH3) and the precipitates analyzed by SDS-PAGE followed by western blotting probed with streptavidin (C). As a loading control, supernatant fractions (see Methods) were analyzed for spectrin content by western analysis (D).
