Fig. 5. Characterization and FRAP measurements of FITC-LTA probe. CHO-CD14 cells were incubated with no FITC-LTA probe (A), 10 ng ml-1 of FITC-LTA probe (B) or 10 ng ml-1 of FITC-LTA in the presence of 100x excess of unlabelled LTA (C). 10,000 cells were analysed for fluorescence on a FACalibur flow cytometer. The histograms display relative cell numbers (y axis) as a function of relative fluorescence intensities (x axis). FRAP curves of FITC-LTA bound to CHO-CD14 (D) ECV-304 cells (E) and Mono-Mac 6 cells (F), measured at 22°C. The best fit to the experimental data is shown. The diffusion coefficient of FITC-LPS bound to cells was the mean and standard deviation from several (
10) individual curves.
