Fig. 1. Co-immunoprecipitation of in vitro translated lamins and emerin. Human lamins A (lamA), B₁ (lamB), C (lamC), human Nup153 (Nup) and emerin were translated as ³⁵S-met labelled proteins in rabbit reticulocyte lysates. Lysates were mixed in the following combinations to give approximately equal starting amounts of radiolabelled protein: (A,B) Emerin+lamin C+Nup; emerin+lamin A+Nup; emerin+lamin B+Nup. (C,D) Emerin+lamin C+lamin B; emerin+lamin A+lamin B; emerin+lamin A+lamin C. (F) Lamin A, lamin B₁, lamin C and emerin translated separately and not mixed. E shows a lower exposure of a lamin A+lamin B+emerin co-immunoprecipitation. The area corresponding to the lamin A and lamin B bands is presented. Two bands are clearly visible. Immunoprecipitations were performed with MANEM3 and -5 in combination (MANEM pull downs (A,C,E)). B and D show starting mixtures. Immunoprecipitates or samples of starting lysates were resolved on 8% SDS PAGE and fluorographed. indicates the position of lamin A; — indicates the position of lamin B; + indicates the position of lamin C; = indicates the position of emerin; ^* indicates the position of Nup153.

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