Fig. 10. Emerin is located in aggregates within the ER in HeLa cells transfected
with GFP-delta 2+. The distribution of emerin was compared with the
distribution of calreticulin in cells that were transiently transfected with
GFP-delta 2+ by four-channel fluorescence. Transfected cells were stained with
DAPI to reveal the distribution of DNA; with MANEM5 (Cy5) to reveal the
distribution of emerin; and with rabbit anti-calreticulin (TRITC) to reveal
the distribution of calreticulin within the ER. Images are displayed either as
individual black and white panels or a four-colour merged image in which DAPI
is displayed in blue, GFP-delta 2+ in green, calreticulin in red, and emerin
in white. It should be noted that when calreticulin staining was omitted no
bleed through between the Cy5 and the TRITC filters was observed. Bars, 10
µm.
