Fig. 6. The distribution of emerin and lamin C in SW13 cells stably transfected
with GFP-lamin A (SW13/20). The distribution of lamin C, emerin and LAP2ß
in SW13 cells was investigated by antibody staining using rabbit anti-lamin C
(Lamin C, row A), MANEM5 (emerin, row B) and LAP17 (LAP2, row C),
respectively. In each case, antibody distribution was compared with the
distribution of GFP-lamin A (GFP). The distribution of DNA in each sample was
detected with DAPI. Each panel displays individual black and white images and
three-colour merged images (merge) in which DAPI is displayed in blue,
GFP-lamin A in green and antibody staining in red. In merged images `yellow'
indicates spectral overlap between red and green signals. Bars, 10 µm.
