Fig. 8. The effects of a dominant negative mutant of lamin B₁ on lamin distribution in HeLa cells. A GFP-fusion of a dominant negative lamin B₁ mutant (GFP-Delta 2+) was transiently transfected into HeLa cells. The distribution of lamin A (row A), lamin C (row B), lamin B₁ (row C) and lamin B₂ (row D) in transfected cells was compared with untransfected cells by antibody staining with JoL2, rabbit anti-lamin C, goat anti-lamin B₁ and LN43, respectively. The distribution of DNA was detected with DAPI. Each panel displays either individual black and white images or three-colour merged images in which DAPI is shown in blue, GFP-Delta 2+ in green and antibody staining in red. Bar, 10 µm.

Return to article