Fig. 9. The influence of GFP-Delta 2+ on the distribution of emerin and LAP2ß
in HeLa cells. HeLa cells were transiently transfected with GFP-delta 2+ and
the distributions of emerin (row A) and LAP2ß (row B) were compared in
transfected and untransfected cells by immunofluorescence using MANEM5
(emerin) and LAP17 (LAP2). The distribution of DNA was detected with DAPI.
Panel displays individual black and white micrographs or three-colour merged
images in which DAPI is shown in blue, GFP-Delta 2+ in green and antibody
staining in red. In merged images `yellow' indicates spectral overlap between
red and green images. Bar, 10 µm.
