Fig. 5. Localisation of TbCLH and TbAPß1 in different life stages. The
far-left panels show PCF cells (A,C) or BSF cells (B,D) probed with anti-TbCLH
(A,B) or anti-TbAPß1 antibodies (C,D). The centre-left panels show DAPI
staining, and the centre-right panels show phase-contrast of the cells. The
position of the antibody labelling relative to the nucleus and the kinetoplast
is shown in the merged panels (far-right). In PCF, TbCLH is localised to
discrete punctata between the nucleus and the kinetoplast but is widely
distributed on large membraneous structures throughout the posterior end of
the BSF. In the PCF, TbAPß1 is present on several small peri-nuclear
vesicles and reticular elements (C) but, by contrast, is concentrated in two
large perinuclear structures in the BSF (D).
