Fig. 6. TbCLH and TbAPß1 closely associate with the Golgi complex, but are on
distinct membranes. (A,B) BSF cells were stained with TbCLH (A, left) or
TbAPß1 (B, left), BODIPY-ceramide (middle), and DAPI (in all panels); the
merged image is shown on the right. TbCLH is observed in structures juxtaposed
to the Golgi complex, whereas the TbAPß1 membranous structure nearest to
the nucleus partially co-localises to the Golgi complex as seen in yellow in
the merged image. (C,D) PCF (C) and BSF (D) cells labelled with rabbit
anti-TbCLH (red) and mouse anti-TbAPß1 (green) and examined by confocal
microscopy. The merged image is shown on the right; the asterix indicates the
position of the nucleus. In neither of these life stages is there significant
coincidence.
