Fig. 9. Ultra thin cryosections showing subcellular localisation of TbCLH. (A)
Conventional Epon section showing clathrin-like coats on a vesicle
(arrowheads) and a tubular profile (arrows). (B-D) Cryosections labelled with
TbCLH followed by 6 nm protein A gold show clathrin in association with a
tubular profile (B); on membranes and vesicles in a region between the Golgi
and the flagellar pocket (C); and also TbCLH labelling of membranes and
vesicles just below the plasma membrane (D). In C, the 6 nm gold particles
originally used for increased resolution have been digitally enhanced using
Adobe Illustrator by placing black circles corresponding to 
15nm
diameter over the Gold particles. Bars, 100 nm, the position of the Golgi
complex, endoplasmic reticulum (ER) and plasma membrane (PM) are
indicated.
