Fig. 10. Anti-VSG antibody traffics through the TbRAB11 compartment subsequent to
internalisation. BSF parasites were incubated with anti-VSG antibody for 1
hour and subsequently processed for fluorescent microscopy. Rabbit anti-VSG
was detected using an anti-rabbit Cy3 conjugate (red). TbRAB11 was detected
using mouse anti-TbRAB11 followed by an anti-mouse FITC conjugate (green).
(A-C) Epifluorescent images; blue, DAPI for DNA. (D-F) Confocal images;
* represents the position of the nucleus. C,F are merged
images.
