Fig. 6. The TbRAB11 compartment is juxtaposed to, but distinct from, early
endosomes and partially colocalises with clathrin-coated structures. (A-C),
Confocal microscopy was used to examine the relationship of TbRAB11 with
TbRAB5A. Cells were stained with mouse anti-TbRAB11 followed by an anti-mouse
FITC conjugate (green) and with rabbit anti-TbRAB5A followed by an anti-rabbit
Cy3 conjugate (red). (D-F) Confocal fluorescent microscopy was used to examine
the relationship of TbRAB11 with trypanosomal clathrin heavy chain. Cells were
stained with mouse anti-TbRAB11 followed by an anti-mouse FITC conjugate
(green) and with rabbit anti-TbCLH followed by an anti-rabbit Cy3 conjugate
(red). C and F represent the merge in each case. Bars, 0.5 µm. *
indicates the location of the nucleus; arrowheads indicate regions of
colocalisation.
