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Fig. 3. Cdc2-YFP leaves the mitotic spindle in early anaphase A, before sister chromatid separation. Time-lapse series showing cdc13-YFP (A, AD112) and cdc2-YFP/cen1-GFP (B, AD265) localisation at the metaphase/anaphase transition. Pictures from cells were taken every 1 or 2 minutes as indicated. Cdc2-YFP fluorescence disappeared from the spindle (B, 2') immediately prior to separation of cen1-GFP dots (arrowheads) on sister chromatids (B, 3'). Bar, 5 µm.





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