Fig. 6. Cdc2-YFP does not leave the mitotic spindle if cdc13p is not recognized by
the APC. Fluorescence of cdc2- and cdc13-YFP was observed in the nucleus and
on the SPBs and spindle of ts mutants defective in either proteasome
(mts2) or APC (cut4-533) function after incubation for 3
hours at 36°C. AD157 (a), AD213 (b), AD152 (c) and AD267 (d). Aggregation
of cytoplasmic cdc2-YFP is observed at 36°C (see
Fig. 4 legend). Upon
overexpression of indestructible cdc13p (lacking the first 81 aa) after 16
hours incubation at 32°C in thiamine-free medium, cdc2-YFP was present in
the nucleus and on the SPBs and spindle of anaphase-arrested cells (AD266, e).
Overexpressed cdc13
81-YFP showed similar localisation (AD217, f). Bar,
5 µm.
