Fig. 7. Association of cdc2-YFP and cdc13-YFP with mitotic microtubules does not
require dis1p function. (A) The endogenous dis1+ gene was
replaced by the dis1-GFP fusion in a cdc25-22 background
(AD219). Cells were arrested at the G2/M transition after 4 hours
incubation at 36°C and dis1-GFP fluorescence was observed in vivo at RT.
Dis1-GFP was found on cytoplasmic MTs in the block (a) and then relocalised to
discrete dots along the forming spindle (b-e). Fluorescence was enriched at
the extremities of the elongating spindle (f-j). c-f show a time-lapse series
of the same mitotic cell as it progresses through mitosis. Bar, 5 µm. (B)
In cold-sensitive dis1-203 cells incubated for 8 hours at 20°C,
cdc2-YFP (AD199, a) and cdc13-YFP (AD264, b) were still associated with the
unsegregated chromosomes, the SPBs and the spindle of mitosis-arrested
cells.
