Fig. 8. Localisation of cdc2-YFP during mating, karyogamy and meiosis. (A) Cartoon
summarizing the localisation of chromosomes, centromeres (
), telomeres
(
) and SPB (
) during mating, karyogamy and meiotic prophase in
S. pombe as described (Chikashige et al.,
1994; Chikashige et al.,
1997). (B) A
cdc2-YFP-expressing strain (AD185) and a cdc2+ strain
(PN745) were crossed on nitrogen-depleted medium and observed by LFM.
Conjugating cells (a-b). Both nuclei become fluorescent (b). During karyogamy,
cdc2-YFP is enriched in bright dots (c-f, arrowheads). In the horse-tail
nucleus, cdc2-YFP is concentrated into 1-3 bright dots (g-k). Nucleus movement
stops and meiosis I starts (l-s). We followed cdc2-YFP fluorescence in the
same diploid cell for 70 minutes from meiotic prophase (i) to metaphase of the
first meiotic division (s). Meiosis I (s-u). Meiosis II (v-x).
