Fig. 9. Cdc2-YFP co-localises with cen1-GFP during meiotic prophase. (A) A
cdc2-YFP-expressing strain (AD185) and a cdc2+ strain
(PN745) were crossed on nitrogen-depleted medium. A time-lapse series showing
cdc2-YFP fluorescence in the horse-tail nucleus of meiotic prophase is
presented (time is indicated in minutes). Cdc2-YFP was found in the
nucleoplasm and was concentrated into 2 or 3 bright dots during nuclear
movement. (B) A cdc2-YFP-expressing strain (AD185) and a cen1-GFP
strain (MKY7A-4) were crossed and a cell in meiotic prophase was observed in
vivo using a laser scanning confocal microscope to distinguish cdc2-YFP (a)
from cen1-GFP (b) fluorescence. In all cases, one cdc2-YFP dot
(arrows) co-localised with the GFP fluorescence associated with the centromere
of chromosome I (arrowheads) (c).
