Fig. 1. Intracellular localization of GFP fusion proteins of Egd1p, Egd2p and
N11-Egd1p. (A) Expression of Egd2p-2GFP in a egd2
strain (to simulate the wild-type situation) and in the NAC-knockout strain.
(B) Expression of Egd1p-2GFP in a egd1 strain (to simulate the
wild-type situation) and in the NAC-knockout strain. (C) Expression of
N11-Egd1p-2GFP in the NAC-knockout strain. (C) Expression of
N11-Egd1p-2GFP in the NAC-knockout strain. Cells were grown to
logarithmic growth phase at 30°C and stained with Hoechst 33258 to
visualize nuclear DNA prior to microscopy. Positions of nuclei are indicated
by arrows.
