Fig. 3. In vitro nuclear import assay of recombinant Egd1p and Egd2p. Recombinant
Egd1p and Egd2p were labelled with fluorescein-5'-maleimide. Dependency
of in vitro nuclear import of Egd1p on different importin 
proteins
(left); import of Egd2p (middle); import of the standard substrate
nucleoplasmin (NPL) by the different importin proteins (right). The
images s, t and u represent the negative controls (import assay without
importin). HeLa cells were permeabilized, the cytosol was washed out
and recombinant import factors, energy mix, and labelled substrate were added.
After fixation the fluorescence of the cells was observed by confocal
microscopy.
