Fig. 5. In vivo transport of yeast NAC proteins into nuclei is inhibited in mutants
for nuclear protein import. (A) Influence of SRP1 mutations on
nuclear import of 
N11-Egd1p-2GFP. srp1-49, srp1-31 and
wild-type cells were transformed with N11-Egd1p-2GFP. Cells were grown
at 25°C (right) and afterwards shifted to 37°C for 3 hours (left).
Position of nuclei are indicated by arrows. (B) Influence of KAP123
and PSE1 mutations on nuclear import of N11-Egd1p-2GFP.
kap123, pse1-1 and the corresponding wildtype were transformed
with N11-EGD1-2GFP. Cells were grown at 25°C and stained
with Hoechst 33258 prior to microscopy.
