Fig. 6. Identification of a domain of Egd1p responsible for nuclear import by
Kap123p and Pse1p. (A) Subcellular location of the indicated N-terminal
deleted Egd1 proteins in a NAC-knockout strain investigated by fluorescence
microscopy. Cells were grown to logarithmic growth phase at 30°C and
stained with Hoechst 33258 to visualize nuclear DNA prior to microscopy.
Positions of the nuclei are indicated by arrows. (B) Influence of
KAP123 and PSE1 mutations on nuclear import of
N14-Egd1p-2GFP. Cells were grown at 25°C. The pse1-1
strain was shifted for 3 hours to 37°C.
